A small secreted protein from Zymoseptoria tritici interacts with a wheat E3 ubiquitin to promote disease.
Identifieur interne : 000223 ( Main/Exploration ); précédent : 000222; suivant : 000224A small secreted protein from Zymoseptoria tritici interacts with a wheat E3 ubiquitin to promote disease.
Auteurs : Sujit Jung Karki [Irlande (pays)] ; Aisling Reilly [Irlande (pays)] ; Binbin Zhou [Irlande (pays)] ; Maurizio Mascarello [Irlande (pays), Belgique] ; James Burke [Irlande (pays)] ; Fiona Doohan [Irlande (pays)] ; Dimitar Douchkov ; Patrick Schweizer ; Angela Feechan [Irlande (pays)]Source :
- Journal of experimental botany [ 1460-2431 ] ; 2020.
Abstract
Septoria Tritici Blotch, caused by the ascomycete fungus Zymoseptoria tritici, is a major threat to wheat production worldwide. The Z. tritici genome encodes many small, secreted proteins (ZtSSP) that likely play a key role in the successful colonisation of host tissues. However, few of these ZtSSPs have been functionally characterised for their role during infection. In this study, we identified and characterised a small, conserved cysteine-rich secreted effector from Zymoseptoria tritici which has homologues in other plant pathogens in the dothideomycetes. ZtSSP2 was expressed throughout Z. tritici infection in wheat with the highest levels observed early during infection. A yeast two-hybrid assay revealed an interaction between ZtSSP2 and wheat E3 ubiquitin ligase in yeast and this was further confirmed in planta using bimolecular fluorescence complementation, and co-immunoprecipitation. Down-regulation of this wheat E3 ligase using virus-induced gene silencing, increased the susceptibility of wheat to Septoria tritici blotch (STB). Together these results suggest that TaE3UBQ likely plays a role in plant immunity to defend against Z. tritici.
DOI: 10.1093/jxb/eraa489
PubMed: 33095257
Affiliations:
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uniqKey="Karki S" first="Sujit Jung" last="Karki">Sujit Jung Karki</name><affiliation wicri:level="4"><nlm:affiliation>School of Agriculture & Food Science and UCD Earth Institute, University College Dublin, Belfield , Dublin, Ireland.</nlm:affiliation><orgName type="university">University College Dublin</orgName><country>Irlande (pays)</country><placeName><settlement type="city">Dublin</settlement><region type="région" nuts="2">Leinster</region></placeName></affiliation></author><author><name sortKey="Reilly, Aisling" sort="Reilly, Aisling" uniqKey="Reilly A" first="Aisling" last="Reilly">Aisling Reilly</name><affiliation wicri:level="4"><nlm:affiliation>School of Agriculture & Food Science and UCD Earth Institute, University College Dublin, Belfield , Dublin, Ireland.</nlm:affiliation><orgName type="university">University College Dublin</orgName><country>Irlande (pays)</country><placeName><settlement type="city">Dublin</settlement><region type="région" nuts="2">Leinster</region></placeName></affiliation></author><author><name sortKey="Zhou, Binbin" sort="Zhou, Binbin" uniqKey="Zhou B" first="Binbin" last="Zhou">Binbin Zhou</name><affiliation wicri:level="4"><nlm:affiliation>School of Biology and Environmental Science and UCD Earth Institute, University College Dublin, Belfield , Dublin, Ireland.</nlm:affiliation><orgName type="university">University College Dublin</orgName><country>Irlande (pays)</country><placeName><settlement type="city">Dublin</settlement><region type="région" nuts="2">Leinster</region></placeName></affiliation></author><author><name sortKey="Mascarello, Maurizio" sort="Mascarello, Maurizio" uniqKey="Mascarello M" first="Maurizio" last="Mascarello">Maurizio Mascarello</name><affiliation wicri:level="4"><nlm:affiliation>School of Agriculture & Food Science and UCD Earth Institute, University College Dublin, Belfield , Dublin, Ireland.</nlm:affiliation><orgName type="university">University College Dublin</orgName><country>Irlande (pays)</country><placeName><settlement type="city">Dublin</settlement><region type="région" nuts="2">Leinster</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Ecology, Evolution and Biodiversity Conservation, Charles Deberiotstraat, Leuven, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Ecology, Evolution and Biodiversity Conservation, Charles Deberiotstraat, Leuven</wicri:regionArea><wicri:noRegion>Leuven</wicri:noRegion></affiliation></author><author><name sortKey="Burke, James" sort="Burke, James" uniqKey="Burke J" first="James" last="Burke">James Burke</name><affiliation wicri:level="4"><nlm:affiliation>School of Agriculture & Food Science and UCD Earth Institute, University College Dublin, Belfield , Dublin, Ireland.</nlm:affiliation><orgName type="university">University College Dublin</orgName><country>Irlande (pays)</country><placeName><settlement type="city">Dublin</settlement><region type="région" nuts="2">Leinster</region></placeName></affiliation></author><author><name sortKey="Doohan, Fiona" sort="Doohan, Fiona" uniqKey="Doohan F" first="Fiona" last="Doohan">Fiona Doohan</name><affiliation wicri:level="4"><nlm:affiliation>School of Biology and Environmental Science and UCD Earth Institute, University College Dublin, Belfield , Dublin, Ireland.</nlm:affiliation><orgName type="university">University College Dublin</orgName><country>Irlande (pays)</country><placeName><settlement type="city">Dublin</settlement><region type="région" nuts="2">Leinster</region></placeName></affiliation></author><author><name sortKey="Douchkov, Dimitar" sort="Douchkov, Dimitar" uniqKey="Douchkov D" first="Dimitar" last="Douchkov">Dimitar Douchkov</name><affiliation><nlm:affiliation>Institute of Plant Genetics and Crop Plant Research (IPK), Cytogenetics , Gatersleben, Germany.</nlm:affiliation><wicri:noCountry code="subField"> Germany</wicri:noCountry></affiliation></author><author><name sortKey="Schweizer, Patrick" sort="Schweizer, Patrick" uniqKey="Schweizer P" first="Patrick" last="Schweizer">Patrick Schweizer</name><affiliation><nlm:affiliation>Institute of Plant Genetics and Crop Plant Research (IPK), Cytogenetics , Gatersleben, Germany.</nlm:affiliation><wicri:noCountry code="subField"> Germany</wicri:noCountry></affiliation></author><author><name sortKey="Feechan, Angela" sort="Feechan, Angela" uniqKey="Feechan A" first="Angela" last="Feechan">Angela Feechan</name><affiliation wicri:level="4"><nlm:affiliation>School of Agriculture & Food Science and UCD Earth Institute, University College Dublin, Belfield , Dublin, Ireland.</nlm:affiliation><orgName type="university">University College Dublin</orgName><country>Irlande (pays)</country><placeName><settlement type="city">Dublin</settlement><region type="région" nuts="2">Leinster</region></placeName></affiliation></author></analytic><series><title level="j">Journal of experimental botany</title><idno type="eISSN">1460-2431</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Septoria Tritici Blotch, caused by the ascomycete fungus Zymoseptoria tritici, is a major threat to wheat production worldwide. The Z. tritici genome encodes many small, secreted proteins (ZtSSP) that likely play a key role in the successful colonisation of host tissues. However, few of these ZtSSPs have been functionally characterised for their role during infection. In this study, we identified and characterised a small, conserved cysteine-rich secreted effector from Zymoseptoria tritici which has homologues in other plant pathogens in the dothideomycetes. ZtSSP2 was expressed throughout Z. tritici infection in wheat with the highest levels observed early during infection. A yeast two-hybrid assay revealed an interaction between ZtSSP2 and wheat E3 ubiquitin ligase in yeast and this was further confirmed in planta using bimolecular fluorescence complementation, and co-immunoprecipitation. Down-regulation of this wheat E3 ligase using virus-induced gene silencing, increased the susceptibility of wheat to Septoria tritici blotch (STB). Together these results suggest that TaE3UBQ likely plays a role in plant immunity to defend against Z. tritici.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33095257</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1460-2431</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Oct</Month><Day>23</Day></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J Exp Bot</ISOAbbreviation></Journal><ArticleTitle>A small secreted protein from Zymoseptoria tritici interacts with a wheat E3 ubiquitin to promote disease.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">eraa489</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1093/jxb/eraa489</ELocationID><Abstract><AbstractText>Septoria Tritici Blotch, caused by the ascomycete fungus Zymoseptoria tritici, is a major threat to wheat production worldwide. The Z. tritici genome encodes many small, secreted proteins (ZtSSP) that likely play a key role in the successful colonisation of host tissues. However, few of these ZtSSPs have been functionally characterised for their role during infection. In this study, we identified and characterised a small, conserved cysteine-rich secreted effector from Zymoseptoria tritici which has homologues in other plant pathogens in the dothideomycetes. ZtSSP2 was expressed throughout Z. tritici infection in wheat with the highest levels observed early during infection. A yeast two-hybrid assay revealed an interaction between ZtSSP2 and wheat E3 ubiquitin ligase in yeast and this was further confirmed in planta using bimolecular fluorescence complementation, and co-immunoprecipitation. Down-regulation of this wheat E3 ligase using virus-induced gene silencing, increased the susceptibility of wheat to Septoria tritici blotch (STB). Together these results suggest that TaE3UBQ likely plays a role in plant immunity to defend against Z. tritici.</AbstractText><CopyrightInformation>© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Karki</LastName><ForeName>Sujit Jung</ForeName><Initials>SJ</Initials><AffiliationInfo><Affiliation>School of Agriculture & Food Science and UCD Earth Institute, University College Dublin, Belfield , Dublin, Ireland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reilly</LastName><ForeName>Aisling</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>School of Agriculture & Food Science and UCD Earth Institute, University College Dublin, Belfield , Dublin, Ireland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Binbin</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>School of Biology and Environmental Science and UCD Earth Institute, University College Dublin, Belfield , Dublin, Ireland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mascarello</LastName><ForeName>Maurizio</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>School of Agriculture & Food Science and UCD Earth Institute, University College Dublin, Belfield , Dublin, Ireland.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Ecology, Evolution and Biodiversity Conservation, Charles Deberiotstraat, Leuven, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Burke</LastName><ForeName>James</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>School of Agriculture & Food Science and UCD Earth Institute, University College Dublin, Belfield , Dublin, Ireland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Doohan</LastName><ForeName>Fiona</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>School of Biology and Environmental Science and UCD Earth Institute, University College Dublin, Belfield , Dublin, Ireland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Douchkov</LastName><ForeName>Dimitar</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Institute of Plant Genetics and Crop Plant Research (IPK), Cytogenetics , Gatersleben, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schweizer</LastName><ForeName>Patrick</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Institute of Plant Genetics and Crop Plant Research (IPK), Cytogenetics , Gatersleben, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Feechan</LastName><ForeName>Angela</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>School of Agriculture & Food Science and UCD Earth Institute, University College Dublin, Belfield , Dublin, Ireland.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Zymoseptoria tritici
</Keyword><Keyword MajorTopicYN="N">E3 ubiquitin ligase</Keyword><Keyword MajorTopicYN="N">disease</Keyword><Keyword MajorTopicYN="N">effector</Keyword><Keyword MajorTopicYN="N">fungal pathogen</Keyword><Keyword MajorTopicYN="N">wheat</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>04</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>10</Month><Day>23</Day><Hour>12</Hour><Minute>11</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33095257</ArticleId><ArticleId IdType="pii">5936541</ArticleId><ArticleId IdType="doi">10.1093/jxb/eraa489</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li><li>Irlande (pays)</li></country><region><li>Leinster</li></region><settlement><li>Dublin</li></settlement><orgName><li>University College Dublin</li></orgName></list><tree><noCountry><name sortKey="Douchkov, Dimitar" sort="Douchkov, Dimitar" uniqKey="Douchkov D" first="Dimitar" last="Douchkov">Dimitar Douchkov</name><name sortKey="Schweizer, Patrick" sort="Schweizer, Patrick" uniqKey="Schweizer P" first="Patrick" last="Schweizer">Patrick Schweizer</name></noCountry><country name="Irlande (pays)"><region name="Leinster"><name sortKey="Karki, Sujit Jung" sort="Karki, Sujit Jung" uniqKey="Karki S" first="Sujit Jung" last="Karki">Sujit Jung Karki</name></region><name sortKey="Burke, James" sort="Burke, James" uniqKey="Burke J" first="James" last="Burke">James Burke</name><name sortKey="Doohan, Fiona" sort="Doohan, Fiona" uniqKey="Doohan F" first="Fiona" last="Doohan">Fiona Doohan</name><name sortKey="Feechan, Angela" sort="Feechan, Angela" uniqKey="Feechan A" first="Angela" last="Feechan">Angela Feechan</name><name sortKey="Mascarello, Maurizio" sort="Mascarello, Maurizio" uniqKey="Mascarello M" first="Maurizio" last="Mascarello">Maurizio Mascarello</name><name sortKey="Reilly, Aisling" sort="Reilly, Aisling" uniqKey="Reilly A" first="Aisling" last="Reilly">Aisling Reilly</name><name sortKey="Zhou, Binbin" sort="Zhou, Binbin" uniqKey="Zhou B" first="Binbin" last="Zhou">Binbin Zhou</name></country><country name="Belgique"><noRegion><name sortKey="Mascarello, Maurizio" sort="Mascarello, Maurizio" uniqKey="Mascarello M" first="Maurizio" last="Mascarello">Maurizio Mascarello</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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